NU 2003-065 

Inventor

Lester Binder*

Short Description

Tau-C3, is a mouse monoclonal antibody (mAb) that binds to human tau truncated at aspartic acid 421

Abstract

Researcher Lester Binder has created a series of useful antibodies that are specific for the human tau protein, pertinent to Alzheimer research. This particular antibody, Tau-C3, is of the IgG1 subtype and would be useful to researchers who wish to detect the truncated form of human tau in tissue and solid phase assay as it has high affinity for the immunogen. It is specific for the caspase-cleaved Tau which is found in a subset of neurofibrillary tangles, tau-containing neuritic plaques, and neuropil threads. Therefore, this antibody is a powerful tool to study the role of truncated tau in Alzheimer's disease and may have implications in the diagnosis of neurodegenerative diseases. The Tau C3 mAb was derived from a hybridoma cell line created through the fusion of SP2/o myeloma cells with splenocytes from a mouse immunized with a peptide corresponding to the C-terminus of tau truncated at aspartic acid 421. The hybridoma cell line was subcloned four times and stored in frozen liquid nitrogen. 

Applications

• Diagnosis of Alzheimer's disease progression
• Research Tool: Protein Analysis ELISA
• Immunoblot
• Immunohistochemistry
• Immunoprecipitation

Advantages

• High Affinity
• High Specific- Only recognizes truncation at D421. Not reactive with full-length or other C-terminal truncations

Publications

Gamblin T, Chen F, Zambrano A, Abraha A, Lagalwar S, Guillozet A, Lu M, Fu Y, Garcia-Sierra F, LaPointe N, Miller R, Berry R, Binder L and Cryns V (2003)  Caspase cleavage of tau: Linking amyloid and neurofibrillary tangles in Alzheimer's disease.  PNAS. 100: 10032-10037. 

IP Status

This antibody is available to be licensed as a research reagent.