NU 1996-011

Inventors

Stephen Adam*

Ermone Adam

Short Description

Antibody for detection of importin from several animal species

Abstract

Northwestern scientists have generated a monoclonal antibody for detection of importin (clone 3E9) from various animal species. This reagent has been validated in several fundamental molecular biology techniques. Antibody 3E9 specifically detects importin in cell lysates (~97kDa). Furthermore, this antibody can be used to inhibit nuclear import, thus enabling research into this fundamental cellular process.

Applications

• Research reagent

Advantages

• Validated for use in Western blots, immunofluorescence and immunoprecipitation experiments
• Can be used for nuclear import inhibition assays

Publications

Chi NC, Adam EJ, Adam SA (1995) Sequence and Characterization of Cytoplasmic Nuclear Protein Import Factor. Journal of Cell Biology. 130: 265-274.