NU 2011-136 and NU 2011-137 

Inventors

William L. Klein*

Pauline T. Velasco

Chi-Hao Luan

Abstract

Northwestern researchers have developed a cortical synaptosome plasma membrane proteome library and a high throughput screening method for determination of novel ligand receptors and receptors antagonist. Ligand-receptor interaction studies are not easy to study on cell-free systems. Further, membrane proteins are difficult to isolate and study without losing their native properties. Northwestern researchers have successfully applied a nanodisc technology, isolating the plasma membrane proteome from a rat cortical synaptic cell line, conferring a unique material to study hundreds or thousands of isolated membrane proteins on a cell-free system. Northwestern researchers have successfully applied this technology to study the interaction between amyloid-derived diffusable ligands with their respective receptors and the detection of antagonists able to block these interactions. 

Applications

• Cell-free ligand-receptors interaction study
• Receptor antagonists high throughput screening
• Library can be labeled for specific protein isolation or placement on different surfaces

Advantages

• Cell-free plasma protein proteasome library system
• Novel ligand receptors discovery
• Compound libraries high throughput screening