NU 2007-060

Inventors

Robert Elghanian

Chad Mirkin*

Short Description

A novel reagent for use in oligonucleotide and related structures, and methods for modifying a wide range of biomolecules in order to attach them to diverse types of surfaces.

Abstract

Northwestern researchers have developed a novel non-nucleoside tosyl phosphoramidite for modification of biomolecules, like oligonucleotides, from both the 3' and 5' ends. The phosphoramidite is prepared conveniently from commercially available starting materials and can be applied to modify molecules post-synthesis or during the synthesis of oligonucleotides to prepare tosylated oligos bearing a single or multiples of tosyl functionalities. The tosyl-modified biomolecules can then be attached on modified surfaces like gold, glass, polymer, metal and plastic.

Applications

• Modification of biomolecules for surface attachment

Advantages

• Modification of oligonucleotides at the 3’ and 5’ ends
• Wide range of biomolecules can be modified – oligonucleotides, polypeptides, lipids and carbohydrates
• Wide range of surfaces can be modified
• Low cost to manufacture

IP Status

Issued US Patent No. 7,829,735